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How do I run FlowJo?
PC and Mac Installers
Choose to place a shortcut on the desktop, and when the installer finishes, double-click on the FlowJo shortcut icon to launch the program. For a Mac, download the . zip file and double-click it to extract the program. Once the zip file is extracted, double-click on the icon to launch FlowJo.
What is FlowJo used for?
FlowJo software is used for the analysis of flow cytometry data. You can use FlowJo to analyze all of your flow cytometry data – regardless of the cytometer used to collect your data files.
Flow Cytometer Basics and FLOWJO Analysis
Images related to the topicFlow Cytometer Basics and FLOWJO Analysis
How do you do a gating in FlowJo?
At the top left of the Graph Window you will see a menu with a horizontal list of icons. Click on the rectangle tool and move your mouse into the graph display. Click-and-drag the mouse to generate a rectangle. Note: once a gate has been set a menu will appear asking you to name the gate.
How do you zoom in FlowJo?
Click the T-button on the axis, select “Customize Axis” and type in minimum and maximum axis values to match the acquisition software. Then click OK. This will allow you to “ZOOM” in on particular sub regions of the data.
How much does FlowJo cost?
An individual copy of FlowJo is normally $2695.00 with the academic discount; the site license brings that cost down to $285/year for each license that can be used on up to 4 different computers (one at a time) or licensed on a shared computer that multiple users can login with their own Portal account to access FlowJo …
Who owns FlowJo?
FlowJo has been acquired as a wholly owned subsidiary of BD, a global medical technology company that is advancing the world of health by improving medical discovery, diagnostics and the delivery of care.
How does FlowJo analyze cell cycle?
FlowJo provides a simple interface to performing fairly sophisticated DNA/Cell Cycle analysis. To launch the univariate cell cycle model click on the population of interest in the workspace, then select the Cell Cycle task from the Biology Band. The univariate model will appear by default as shown in Figure 1.
Why do we use flow cytometry?
Flow cytometry may be used whenever your healthcare provider needs to learn more about the cells inside your body. This type of testing can check the number of immune cells, assess your cell cycle status, identify cancer cells or even analyze your DNA.
What is Biexponential transformation?
Biexponential scaling helps visualize data that is compressed against the low x- and y- axes. “Squished” data is easily viewed by adding a section of linear scale to log acquired data.
How do I make a table in FlowJo?
In the Table Editor, type in the new names in the Column Name column. When you create a table, these names will be displayed instead of FlowJo’s statistic name. Drag and Drop, Copy and Paste You can drag a table or copy it from the Layout Editor in one Workspace into the Layout Editor of another.
FlowJo vX Lessons 4 Gating and Statistics
Images related to the topicFlowJo vX Lessons 4 Gating and Statistics
What is mean fluorescence intensity?
Mean Fluorescent Intensity (MFI) is often used to compare expression of target of interest (TOI) across samples/ cell populations in Flow cytometry. It gives reliable information about expression/ presence of TOI within the experiment.
What is a layout editor?
Layout editors are primarily responsible for designing the format of publications, such as magazines, newspapers, books and online materials. They utilize layout software to assemble text, photographs and other content in an aesthetically pleasing and easy-to-read arrangement.
How do you draw a line in FlowJo?
The Line/Arrow tool is used for drawing simple lines and arrows in the Layout Editor. Select this tool then click and drag anywhere in your layout to draw a line. To resize it, click on one of the square handles at the ends of the line. To move the line, move the mouse over the line or arrow, watching the cursor.
How do I change the color of my gate in FlowJo?
To view or change your gating preferences, click the preferences icon represented by a red heart outline on the workspace toolbar. Gate Settings: The gate settings determine the default appearance of gates in graphs. You can set gates to tinted, change gate color, and change the gate line weight.
How do I change the Y axis scale in Flowjo?
You can quickly change the scale of any parameter to logarithmic or linear by clicking the “T” button and selecting “Log Axis” or “Linear Axis” from the drop-down menu, respectively. Use the “Reset” option to change your axis back to its default setting.
What is FlowJo dongle?
A Dongle is a perpetual license for FlowJo v10 or v9. A Dongle plugs into USB port on either a Mac or a PC and can be used at multiple workstations (one at a time). Buy Now > FlowJo Portal. FlowJo Portal is our user-based licensing system for FlowJo annual subscriptions.
What is FlowJo v10?
Welcome to FlowJo, a software application with an integrated environment for viewing and analyzing flow cytometric data.
Who is FlowJo?
Florence Delorez Griffith Joyner (born Florence Delorez Griffith; December 21, 1959 – September 21, 1998), also known as Flo-Jo, was an American track and field athlete. She set world records in 1988 for both the 100 m and 200 m.
Intro to FlowJo v10 with Jack 9.7.17
Images related to the topicIntro to FlowJo v10 with Jack 9.7.17
What is FACS analysis?
Fluorescence activated cell sorting (FACS) analysis is a derivative of flow cytometry that proceeds in a slightly different direction. The primary objective of FACS flow cytometry is to physically sort a heterogeneous cell sample into separate populations. Isolated cells can then be used for further research.
What is flow cytometry software?
Flow cytometry software is the key interface between the researcher and the instrument, so meeting your requirements and being easy to use is important. Streamline your sample acquisition and data analysis with the NovoExpress Software. One software combines sample acquisition and data analysis.
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