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How do you make a PEG solution?
PEG/NaCl (5x) stock solution: PEG-8000 20%, NaCl 2.5 M. Dissolve 100 g PEG-8000 (20% w/v) and 75 g NaCl (2.5 M) in 400 ml ddH20 and bring to a final volume of 500 ml by stirring at RT. The solution can be autoclaved (optional) but mixing during the cooling period is required to prevent a phase separation.
How do you dissolve PEG 6000?
PEG can be dissolved in warm water at 80-90 °C with no adverse effects. Sterile filtration of the solution is recommended using a 0.45 µm filter, initially. Although autoclaving of PEG in saline solutions has been reported,4 it is not recommended.
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How do you make a 20 PEG 6000 solution?
You can make a single solution containing 20% PEG and 2.5M NaCl. Since NaCl is easily soluble in water, dissolve the calculated amount of NaCl in water. Weight the required amount of PEG 6000 and add small quantities of PEG in the above solution and vortex vigorously to dissolve it.
How do you dissolve PEG 8000?
To rapidly dissolve the PEG 8000, the suspension can be briefly microwaved for no more than 15 sec with the unscrewed lid placed loosely on top of the conical tube. (Do not attempt to microwave the tube with the lid screwed on.)
Can PEG 6000 be autoclaved?
PEG can be dissolved in warm water at 80-90 °C with no adverse affects. Sterile filtration of the solution is recommended using a 0.45 µm filter, initially. Although autoclaving of PEG in saline solutions has been reported, it is not recommended.
How do you make a 40% PEG?
For a 40% w/v solution, dissolve 40g of PEG 8000 in water or buffer and adjust the volume to 100 mL. For a 40% w/w solution, mix 40g PEG 8000 with 60g water or buffer until you have a solution. Usually in crystallization the %(w/v) annotation is used so that would be 20 g dissolved to an end volume of 50 ml.
What is polyethylene glycol PEG 6000?
Poly(ethylene glycol) 6000 is a high quality research product used as multipurpose polymer of ethelyne glycol for various biochemical, molecular biology and molecular diagnostic applications.
Does PEG dissolve in water?
How do you dissolve PEG 3350 in water?
Add lukewarm water to the fill mark on the bottle. If you are using flavoring powder that comes with the medicine, add it before adding the water. Do not add any extra flavorings or starch-based thickeners to the medicine. Shake well until all the ingredients are dissolved.
What is peg8000?
PEG 8000, Molecular Biology Grade (Polyethylene Glycol 8000) 500g. $ 79.00. PEG 8000 is used in the precipitation of phage, isolation of plasmid DNA and the enhancement of blunt-ended ligation reactions. Formula Weight: 7,000–9,000.
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Is PEG 8000 soluble in water?
Solubility : Soluble in water (500 g/l at 20° C), aromatic hydrocarbons (very soluble), aliphatic hydrocarbons (slightly soluble), and organic solvents.
What is PEG soluble in?
What is PEG solution?
Polyethylene glycol (PEG) and electrolytes combination solution is used to cleanse the colon (bowel) before certain medical tests (eg, colonoscopy, barium enema X-ray exam) or colon surgery. PEG-electrolyte solution is a laxative. It works by causing you to have diarrhea to clean your colon.
How do you filter PEG solutions?
Make up PEG (MW 350) to 50% (w/v) with H2O and filter-sterilize with a 0.45-µm filter unit (Nalgene). Alternatively, the PEG solution can be autoclaved, but it must be at the proper concentration. Store the PEG in a tightly capped container to prevent evaporation of H2O and a subsequent increase in PEG concentration.
Can you autoclave PEG 8000?
Autoclaving PEG is not recommended (See Sigma Specs), I am assuming that having your PEG at 8000mw is important for your experiment, and if you autoclave it, it will not be anymore. we routinely make up 24% PEG 8000 for virus precipitation and it filter sterilizes just fine at this concentration.
Is PEG light sensitive?
PEGs and PEG derivatives are sensitive to light and oxidation. Therefore, for both long time and short time storage, all PEGs and PEG derivatives should be stored under Nitrogen or Argon and in the dark.
How do you make a 50% PEG 4000 solution?
- Weigh 50g PEG4000.
- Dissolve this by adding 50ml H2O and warming it if needed.
- Top up to 100ml.
- Filter sterilize.
- Make 1.5ml aliquots and store these at -20C.
How do you make PEG 50%?
- Measure out 25 grams of PEG 3500 into a 100 mL glass bottle.
- Add 25 mL water and vortex well. Let rest for 10 min. …
- Add water to 50 mL total volume. Vortex again and let rest until solution looks clear. …
- Sterilize by autoclave on liquid 20 minute cycle or sterile filter into a new bottle.
What is the difference between PEG 6000 and PEG 8000?
peg6000 and 8000 are the same, peg6000 was found to have an average fw of 8000 and was renamed. The clue is in the name – POLYethylene glycol!
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How do you make polypropylene glycol?
Polymerization. Polypropylene glycol is produced by ring-opening polymerization of propylene oxide. The initiator is an alcohol and the catalyst a base, usually potassium hydroxide. When the initiator is ethylene glycol or water the polymer is linear.
How is PEG made?
How is PEG Made? Like most polyethers, polyethylene glycol is made by polymerising (combining with) monomers. It does this by forming ether links between them, which have a chain-like molecular structure. In the case of PEG, ethylene oxide is reacted with ethylene glycol.
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